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J Korean Bal Soc > Volume 1(2); 2002 > Article
Journal of the Korean Balance Society 2002;1(2): 223-234.
흰쥐에서 말초전정기관 손상이 해마의 cFos 및 FosB 발현에 미치는 영향
김민선, 최명애, 최동옥, 조병곤, 김원철, 김재효, 다비 크라이, 박병림
원광대학교 의과대학 생리학교실
Effect of vestibular end-organs on expression of cFos and FosB injury in the hippocampal formation of rats
Min Sun Kim, Myoung Ae Choi, Dong Ok Choi, Byung Gon Cho, Yuan Zhe Jin, Jae Hyo Kim, Davy Kry, Byung Rim Park
Department of Physiology, Wonkwang University School of Medicine, and Vestibulochlear Research Center at Wonkwang University, Iksan 570-749, Korea. byungp@wonkwang.ac.kr
ABSTRACT
It is well known that the hippocampal formation requires primary vestibular sensory information to generate spatial memory during self motion in human. The purpose of the present study was to evaluate the effect of unilateral deafferentation of vestibular sensory information on cFos and FosB proteins, a family of immediate early gene-related proteins known as metabolic marker for neural excitation in the hippocampal formation of rats. Adult Sprague-Dawley rats weighing 250 - 300 g were surgically ablated of the peripheral vestibular system in the inner ear and sacrificed at 2, 6, 24, 48, 72 hours after surgical operation. Immunohistochemical staining and Western blot method were adapted to see change in expression of cFos and FosB proteins in the hippocampal formation. A significant change of Fos B immunoreactivity was observed in granular cell layer of the dentate gyrus, CA1 subfield of the hipocampus at 2 hours after unilateral labyrinthectomy. Thereafter, the number of FosB like immunoreactive neurons in these areas increased rapidly, peaked at 48 hours post operatively time. Western blot for FosB protein supported further time-dependent change of FosB revealed by immunohistochemical staining. In addition, granular cell layer showed more significant expression of FosB LI neurons in the caudal dentate gyrus than the rostral one. In contrast, moderate number of cFos LI neurons was detected in polymorphic cell layer of the dentate gyrus, pyramidal cell layer of CA1, and subiculum but not in granular cell layer of the dentate gyrus at 2 hours after labyrinthectomy. The number of cFos LI neurons in the hippocampal formation was rapidly decreased at 6 hours and then returned to basal value 24 hours after operation. These results suggest that unilateral ablation of the peripheral vestibular sensory information elicit spatio-temporal differences of cFos and FosB expressions in the hippocampal formation of rats.
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